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Collected diatom samples were enumerated to 250 valves and identified to species or subspecies.
Microbial cells in the formaldehyde-preserved samples were enumerated using direct microscopic counts.
Samples were enumerated by a 10-fold dilution series, plating each dilution onto TSA, and counting the colony forming units (CFU) after sufficient growth at 37°C overnight.
Internalized Salmonella spp. in betel leaf samples were enumerated and result showed that a significant portion of total Salmonella spp. in the leaves are internalized.
The inoculated samples were enumerated up to 15 days at every 24-h by means of standard spread plate methods (Cappuccino and Sherman 1996; Feroz et al. 2013).
Samples were enumerated in an ELISPOT reader (Cellular Technology, Shaker Heights, OH) and the results shown as the number of ASC per 106 cells.
Similar(49)
The positive species for each sample were enumerated.
For quantitative analyses, percentages of apoptotic cells per ⩾200 counted per sample were enumerated.
Oocysticidal property was evaluated in term of sporulation inhibition of oocysts and the percentage of unsporulated, sporulated and degenerated oocysts, after treatment with 125 μg/ml of the selected essential oil, the sample was enumerated by haemocytometer, while coccidiocidal activity was assessed by the inhibition of sporozoite invasion in MDBK cell lines.
Each sample was enumerated in triplicate.
The plates were incubated in a humidified incubator for up to 24 hours and the bacterial density from each sample was enumerated visually.
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