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167 patient samples were entered into the study, consisting of 85 frozen and 82 fresh isolates.
All 21,671 genes for each of the samples were entered into the analysis.
A total of 154 blood samples were entered into the study.
If the SD's were approximately constant over the measured range, the mean of SD's of those quality control samples were entered.
For this validation step, tumour and normal samples were entered blindly into the discriminatory model in order to determine sensitivity and specificity.
Sequence data from the giraffe samples were entered in the GenBank database under the following Accession numbers [GenBank: EU548032 and GenBank: EU548033].
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Mean IQ of samples was entered as dependent variable and assumed year of study performance served as predictor.
Time points of blood sampling were entered into the model as a factor, liver response to treatment at 1-month follow-up was entered as a fixed effect (dichotomous as response: complete response (CR), partial response (PR) or stable disease (SD); no response: progressive disease (PD)), and random intercepts were used for the individual patients (independent data).
The fields included in the database are presented in Table 2. Laboratory results and field information collected during the sampling were entered into the database.
To determine the appropriate housekeeping gene to be used to normalize the data, the Cq for GAPDH and β-actin, and all target genes per sample were entered into the BESTKEEPER program.
Once a sample is entered into a group it cannot be extracted again (i.e. hierarchical).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com