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Once the samples were dry in the desiccators, the surface-bound T cells were sputter-coated with platinum before the SEM measurement was performed.
After the 2-week period, samples were removed from the saline solution using pre-cleaned tweezers and placed on labeled microscopic glass slides under a fume hood until the samples were dry.
[10] Kamei, G., and Ohmoto, H. (2000) 105 – 150 μm Samples were dry ground in two steps: 1) a glass-cleaned ring pulverizer was used to reduce grain size and 2) an agate mortar was used to crush the particles to the desired particle size range.
Samples were dry in 1 3 days and all dried satisfactorily.
The tubes with the lids off were weighed and then dried in a SpeedVac (Thermo Savant, Waltham, MA) for 4-6 hountilntil all samples were dry.
Samples were placed under a stream of high purity dry nitrogen gas until all the samples were dry.
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The digested samples were dried in a vacuum centrifuge.
The samples were dried structurally characterised.
The samples were dried to constant weight and their biomass was weighed.
The tissue samples were dried at 60 °C in a gravity convection oven until reaching constant weight.
Samples were dried by conditioning in a desiccator for several days.
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