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All samples were dispensed in one sequence over no more than 10 min using a single batch of cells in suspension.
The beads were washed standard, control and samples were dispensed into the plate.
The frozen samples were dispensed in 0.2 g aliquots into 4 mL WISP style screw thread amber glass vials and sealed with Black Top Hat PTFE/Silicone caps (J.G. Finneran, Vineland, NJ).
Next, the fragmented resuspended DNA samples were dispensed on BeadChips.
Biological samples were dispensed into aliquots and frozen and stored at -40°C until analysis.
Resin-cleaned samples were dispensed to a 384 SpectroCHIP preloaded with matrix (SEQUENOM, Inc., San Diego, CA, USA) by Nanodispenser.
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Approximately 1 g (dry basis) of the samples was dispensed into 200 mL Erlenmeyer flasks.
At this point, samples are dispensed into 1.4-mL 2D Tracker U bottom Micronic tubes (MP52607PK, Micronic North America, McMurray, PA) that were scanned and weighed on iWALL.
However, that assumption may not be true in clinics where free drug samples are dispensed, wherein there may be a gap of weeks to months between the office visit when treatment was started and the first ICS/LABA prescription drug fill, resulting in a bias in follow-up time.
Triplicates of each sample were dispensed into 96-well plates and incubated at 37°C.
At each time point, 2 aliquots (20 µl each) of the sample were dispensed into white 96-well assay plates (White w/Lid, Tissue Culture-Treated, BD Biosciences, San Jose, CA).
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CEO of Professional Science Editing for Scientists @ prosciediting.com