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The samples were disaggregated to single cell suspensions by shaking prior to use for pigment determination.
Finally, the samples were disaggregated and homogenized before being sieved to 2 mm (soil metal concentration analysis), and then stored at ambient temperature (t = 22°C) for further analysis.
Biopsy specimens from tumor and adjacent normal tissues were collected from two colorectal cancer (CRC) patients (pt 78 and pt 80), samples were disaggregated by enzymatic treatment and the epithelial cells affinity purified.
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After drug treatment, half of the spheroids (control and treated; 4 for each sample) were disaggregated by trypsin without chelators for fifteen minutes at 37 degrees and re-seeded as single cell suspensions on flat bottom 96 well plates for conventional 2D culture.
2) Once the sample is disaggregated, wash it over a fine mesh sieve (240 m) to remove all the silt and clay (you can use a 125 m mesh sieve but the smallest forams will be lost).
Within each of these blocks, the Control sample was disaggregated into three performance groups and the associated RT distributions were compared numerically and graphically.
The specimens were heated in air and in a vacuum from room temperature up to 700 °C in a field of 300 mT. The rock samples for the oxygen isotopic analysis were disaggregated primarily using Na2SO4 and partly using a high-voltage pulse power fragmentation system (SELFRAG Lab SELFRAG AGG, Switzerland) installed at the NIPR.
The gouge sample was made from the core blocks that were disaggregated and sieved to small grains.
The biopsy specimens were disaggregated mechanically and primary fibroblast cultures were initiated by an outgrowth technique in which skin samples were incubated in complete growth media (see below) in a humidified atmosphere with 5% CO2 in air at 37°C.
Next, EBs were disaggregated and re-plated in piPSCs growth conditions for 7 days.
Once confluent, cells were disaggregated from each flask using trypsinisation.
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