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Samples were digested with digestion solution in fume hood.
For this process, samples were digested with HNO3 for 2 h in a digester block between 90 and 120 °C, using the open system with the cold finger.
Reduction and alkylation were performed in-solution, and samples were digested with trypsin overnight.
The samples were digested with aqua regia and analyzed by ICP-MS.
Samples were digested with perchloric acid and nitric acid.
The samples were digested with DNaseI (Takara, Shiga, Japan).
The AOA and AOB samples were digested with restriction with HpyCH4V and MspI, respectively.
Prior to AAS analysis, water samples were digested with 5 mL of concentrated HNO3 aq).
Subsequently, the samples were digested with the microwave oven using a two-step temperature program.
Subsequently, dried samples were digested with trypsin by incubation overnight at 37°C.
The samples were digested with sequencing grade trypsin (Promega) inratio 1 100 w/w (trypsin: protein) overnight at 37°C.
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