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After washing with NTMT buffer and PBSS, the solution was exchanged to 100% ethanol in two steps (50, 100%), and the samples were de-coloured for 1 h.
Selected bulk rock samples were de-oiled, disaggregated and powdered to a mean particle size between 5 and 10 µm for the XRD analysis.
The filtered samples were de-fatted by adding 0.5 mL of n-hexane/dichloromethane (3:2, v/v), shaking for 10 s, and removing the upper organic solvent.
All samples were de-identified and coded at the time of sample collection, and the laboratory remained blind to the study group and family affiliation.
Written consent was received and all samples were de-identified before analysis.
Because the samples were de-identified and were considered as samples normally discarded, the committee felt that there was no specific patient consent necessary.
All samples were de-identified and were either available from BIDMC or CHB, or were purchased from Elixir Pharmaceuticals or NECS for a processing fee.
For immunofluorescence staining, samples were de-paraffinized, hydrated and subjected to antigen retrieval by boiling in 10 mmol/L sodium citrate (pH 6.0) for 10 min in microwave followed by 30 min gradual cooling at room temperature.
Exemption from IRB approval was granted by the Uniformed Services University of Health Sciences IRB on the grounds that specimens were obtained via informed donation from cadaveric material in accordance with federal and state regulations, the research did not encompass genetic linkage studies, and all samples were de-identified and personal information anonymised.
The samples were de-identified by pooling.
Prior to the study, samples were de-identified.
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CEO of Professional Science Editing for Scientists @ prosciediting.com