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Reactive samples were confirmed using Western blot I and II confirmation kits (New Lav-Blot I and II; Bio-Rad Laboratories).
The identities of all tumour and normal DNA samples were confirmed using mass spectrometric fingerprint genotyping of 24 common single-nucleotide polymorphisms (SNPs; Sequenom, San Diego, CA).
Repeatedly reactive samples were confirmed using the Genetic Systems Confirmatory Assay 3.0 (BioRad Laboratories, Redmond, WA).
Syphilis serology was conducting using the rapid plasma reagin (RPR) test (Macro-Vue, Becton Dickenson, Cockeysville, MD, USA); RPR-positive samples were confirmed using the Treponema pallidum particle agglutination assay (TPPA) (Fujirebio, Wilmington, DE, USA).
The genotypes of 19 samples were confirmed using direct sequencing.
Infected samples were confirmed using viral titres (data not shown).
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The change of ionic association in the scCO2-treated samples was confirmed using FT-IR measurement.
The presence of virus in the samples was confirmed using one-step q-PCR that targeted a conserved region of the avian influenza A matrix gene.
The presence of anti-core+1/ARFP antibodies in these plasma samples was confirmed using ELISA test with synthetic core+1/ARFP peptide encompassing aa sequence 91 106 of the protein (data not shown).
The absence of cartilage degeneration in the normal cartilage samples was confirmed using Safranin-O staining.
The quality of purified RNA samples was confirmed using Experion (BioRad).
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CEO of Professional Science Editing for Scientists @ prosciediting.com