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Discrepant samples were confirmed as true positives using a probe-based real-time quantitative PCR assay.
Only 46 samples were confirmed by the AxSYM® and the VIDAS® assays.
The chemical states of Ti in the samples were confirmed by X-ray photoelectron spectra (XPS).
Positive samples were confirmed by western blotting.
Positive samples were confirmed by Western blot assay.
The identification and purity of the samples were confirmed by SDS-PAGE (12% gel).
All positive samples were confirmed by a second PCR reaction from the remaining cDNA.
After tested by RT-PCR, 149 samples were confirmed to be positive for H5N1 subtype.
The histological diagnoses of all samples were confirmed by an experienced pathologist.
Repeatedly reactive samples were confirmed using the Genetic Systems Confirmatory Assay 3.0 (BioRad Laboratories, Redmond, WA).
All samples were confirmed histologically.
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