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The filtered seawater samples were circulated through the closed loop experimental setup for durations of seven and 14 days and the fouling thermal resistance was measured continuously.
To ensure saturated binding of the recombinant σS to the matrix, samples were circulated through the column for 2.5 h using the Akta Explorer system (Amersham), and then washed extensively with Buffer A until the OD280 of the eluate dropped to a baseline reading.
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The retenate was centrifuged at 4000 g for 20 min and the sample was circulated on an affinity column overnight.
Before an experiment, the sample was circulated at a set temperature for 10 min to ensure temperature equilibration.
Clearly defined sampling guidelines were circulated to all partners prior to sampling to ensure that only samples of known origin were included in the study and to avoid any contamination of samples.
After RNA hybridization, tag-conjugating Cy3 dyes (one-color hybridization) were circulated to samples for dye staining.
A sampling of his ad hominem remarks were circulated some weeks ago [ the July 19th issue] in The Times Literary Supplement, in an item meant to show up Naipaul's imperial dismisiveness (he is referred to, with heavy-handed irony, as "the great man").
Imperial orders for his arrest were circulated.
Petitions were circulated.
The high number of trypanosomes positive serum samples suggests that the trypanosomes were circulating in the hemolymphatic system.
T-shirts were circulating that read: "Nixon.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com