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Of the 145 control women who gave initial consent, cord samples were attempted in 75 and obtained in 70.
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For comparison, the synthesis of PbTe samples was attempted with water as the solvent.
Introducing the reduction of grain sizes into Y x Al y B14 samples was attempted by two methods; one was through mechanical grinding, and the other was by synthesizing Y x Al y B14 via Y0.56B14 (denoted as "vYB-YAlB14").
Initial isolation of STEC from stool samples was attempted at the primary diagnostic laboratories.
RVFV isolation from mosquitoes and human samples was attempted by inoculation in AP61 cell lines, followed by indirect immunofluorescence assay, as described (12 ).
Molecular subtyping of the 7 pla-positive DNA samples was attempted by clustered regularly interspaced short palindromic repeats analysis, targeting the 3 loci YPa, YPb, and YPc, respectively.
Virus isolation from positive samples was attempted by inoculation of nasal swabs samples into specific pathogen free embryonated chicken eggs (ECE) and complete genome sequencing was performed on virus strains after replication on ECE or from original swab sample.
Virus isolation from porcine samples was attempted on Vero V76 and porcine turbinate cells PT-K75, seeded at a density of 2 x 10 cells/cm in 12-well plates (Costar) 24 h before inoculation.
In our earlier study, evaluation of antibody responses to CSE antigen in PT serum samples was attempted in 10 NCC patients who reported 1, 3, and 6 months following albendazole treatment (15 mg/kg body weight for 15 days).
In addition, virus isolation from chicken samples was attempted on chicken embryo epithelial kidney cells, seeded at a density of 10 cells/cm in 24-well plates (Costar), using CPE format followed by RT-PCR.
Though the survey was cut short by the approaching storm, all numbers in the sample were attempted at least once.
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