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After ubiquitination, the samples were applied to an SDS polyacrylamide gel, followed by western blotting with anti-T7 antibody.
Then, the samples were applied glaze for trial purposes.
For negative staining, 3 μl samples were applied to glow-discharged carbon-coated copper grids, stained with 2% (w/v) uranyl acetate and air-dried.
Student's t-test analyses (for paired samples) were applied to determine the statistical significance values defined as NS: P>0.05; *P>0.05; **P>0.05; ***P>0.051.
The same procedures for the as-is samples were applied.
Samples were applied using a Micro dispenser (DRUMMOND).
The samples were applied to SDS-PAGE and Western blot as described (Nguyen and Schumann 2006).
Samples were applied to the glow-discharged grids and adsorbed for 1 min at RT.
The samples were applied to a 0.8% (w/v) agarose gel in TAE buffer as described above.
Triplicate samples were applied to the array.
Duplicate samples were applied to the arrays.
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