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For validation of proposed system performance, entire samples were again sorted by experts visually.
After cooling in a desiccator, crucibles with samples were again weighed and ash content calculated.
Once they had been conditioned, the samples were again checked using the BonNDTinspect system.
Before AFM and SICM experiments, the treated cell samples were again washed three times with PBS.
The samples were again dried at 100°C until attaining a constant weight.
The samples were again vortex mixed gently for 1.5 min and then cold centrifuged for 10 min at 10000 rpm.
After one month of cultivation of spinach, soil samples were again taken from the plots (04 August).
Finally, the samples were again placed in a humidity oven, and after drying, their porosity and permeability were measured.
When removed from the solution, the samples were again rinsed in deionized water and dried in nitrogen gas flow.
Samples were again centrifuged (14,000 rpm, 20 min, 4 °C), the supernatant transferred into a fresh tube and stored at −20 °C.
The samples were again briefly vortexed and then allowed to stand at room temperature for 5 min before measuring the glycogen content.
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