Sentence examples for samples were added with from inspiring English sources

Samples were added with loading buffer (consists of β-mercaptoethanol and SDS), denatured at 99°C for 10 min and equilibrated to room temperature, and then 50 or 100 μg of proteins were subjected to 12.5% SDS-PAGE and transferred to a nitrocellulose blotting membrane with a Pyxis machine (GE Healthcare, USA).

For hydroxylamine cleavage or proteolytic digestion experiments, the labeled samples were added with DTT to a final concentration of 40 mM after labeling.

Aliquots of samples were added with an equal volume of isopropanol/butanol (1∶1 v/v) and centrifuged at 20000 g for 5 min. Pellets were re-suspended in NuPage LDS Sample Buffer (Invitrogen) and were analysed by Western Blotting.

Samples were added with a combination of three detergents, 0.5% digitonin, 0.2% sodium cholate and 0.5% NP-40 (final concentration), which would be henceforth referred to as triple detergent, and the samples were left for end over end shaking for 60 min in 4°C.

Cells were washed with 1 ml of sterile water and resuspended in 1 ml of cold 0.25 M NaOH/1% 2-mercaptoethanol, followed by incubation on ice for 10 min. Samples were added with 160 µl of 50% TCA and incubated on ice for additional 10 min. Cells were pelleted at full spin for 10 min and then washed with 1 ml of acetone.

Samples were added with assay diluents.

About 0.5 g of the ground soil samples was added with 2.0 ml of HClO4, 2.0 ml of HCl, 8.0 ml HHNO3 and 2.0 ml HF.

1.5 mL of the prepared samples was added with 3 mL dichloromethane (Merck, Darmstadt, Germany) and immediately after acidifying with 100 μl 0.5 mol/l sulphuric acid was moderately shaken for 20 s.

In brief, 12 μl RNA from each sample were added with 2.5 μM Oligo (dT 20 primer and 0.5 μM dNTPs (10 mM), incubated at 65°C for 5 minutes (min) and kept in ice for at least 1 min; 4 μl 5 × SuperScript™ III Reverse Transcriptase First-Strand Buffer, 5 mM DTT (0.1 M), 20 Units of RNase OUT, and 200 Units of SuperScript™ III Reverse Transcriptase (200 u/μl) were added to the reaction.

Each tea sample was added with 90 mL normal saline and homogenized for 3 min.

Each sample was added with 80 μl 6N hydrochloric acid and boiled for 10 min. After cooling in a water bath at room temperature, the solutions were colorized with 80 μl 5M KSCN, and their A480 were measured.