After incubation, samples were added to cells and residual luciferase expression was measured after 24 h.
Serially diluted samples were added to the wells in triplicate and incubated overnight at 4 °C.
Moreover, some samples were added lithium carbonate to reduce the setting time for the cement mixture.
These new annotated samples were added to the initial 171, adding up to 547 samples belonging to 528 patients.
Following data collection without phage, the RNA samples were added to prepared Pf1 phage (Cederlane, P-200P).
Samples were added 1 h before incubation with LPS.
Two microlitre of DNase I solution (concentration series) or serum samples were added to the wells.
For photometric analysis applying Ehrlich's Reagent, the samples were added to an equal amount of conc.
The samples were added to water to achieve concentrations of 500 to 3,000 ppm.
Subsequently, different dilutions (50 μl) of freshly prepared samples were added to each well.
Then serum samples were added and plates were washed again.
