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For amplification of DNA extracted from eggshell samples, we designed a new pair of primers using Primer3 (ref. 28) to amplify a short, 159-bp region of COI.
To make the systems more useful in analyzing degraded DNA samples, we designed primers to render amplicons of ⩽150 bp.
To make the systems more useful for analyzing degraded DNA samples, we designed primers to render amplicons of 100 bp or shorter (shorter PCR products).
In order to develop a forensic molecular clock for post-mortem blood samples, we designed a study to identify circadian markers in three different classes of circadian molecules using post-mortem blood samples obtained from deceased individuals with a known time of death: mRNA transcripts using RNA-Seq, metabolites with LC MS/MS and hormone concentrations using ELISA.
Due to some difficulty with very weak false positive results obtained using the commercial detection kit with some samples, we designed an alternative, nested RT-PCR detection method with specific primers to target the viral RdRp region instead of the capsid gene targeted by the commercial kit.
As part of a larger effort to develop lab-on-a-chip methods for detecting radiation exposure events using blood samples, we designed a dose course microarray study in order to determine coding and non-coding RNA transcripts undergoing differential expression immediately following radiation exposure.
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Using two-stage (schools and students) sampling, we designed questionnaires based on pupils' school commuting patterns and collected the data for the two groups.
Then, for obtaining the effective samples, we design a new hybrid sampling mechanism, which can sample the local and global predicted location according to confidence map.
To reflect such a difference between the samples, we elaborately design an independent soft label for each sample of each class rather than a common label for all the samples of the same class.
To validate a sample of detected SNPs, we designed primers to amplify 500 700 bp of transcripts containing a large number of putative SNPs.
In order to accurately measure the X-ray flux and determine the dose absorbed by the sample, we have designed an active beamstop device.
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