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After coring in these two directions of rock samples, we conducted Triaxial Compression Tests using Changchun Sunrise TAW-1000 Deep Water Pore Pressure Servo-system, and its stress strain curves are shown in Fig. 2.
To understand population differentiation within T. cacao and to overcome the problem of mislabeled samples, we conducted a study using Bayesian statistics implemented through the software Structure [12].
Since the cDNA comparisons could only include the two populations for which we have preserved RNA samples, we conducted an expanded genomic DNA survey to determine if there were any allele frequency differences in these ABP genes across the species' range in Alaska.
To address the question whether the set of 179 genes together and the two sets of 149 and 30 genes separately were significantly enriched in this set of tumor samples, we conducted a gene set enrichment analysis (GSEA) as described by Subramanian [34].
For both study samples, we conducted bivariate analyses examining the reported relative activity level as a function of age decade.
Regarding the first two groups as normal, and the last two groups as cancer samples, we conducted enrichment and association analyses.
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To ensure the thoroughness of the sample, we conducted internet searches to identify additional institutions using SCALE-UP instruction.
In the process of Gibbs sampling, we conducted a total of 40,000 iterations on the parameters, to ensure the convergence of parameters.
For each sample, we conducted three biological and three technical replicates.
After we had recruited about half of the sample, we conducted an interim analysis of the data.
Before sampling, we conducted 5 to 10 minutes of flushing using the well water to remove any sandy water.
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