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Differential expression of these genes in either Cr_large or Cr_small samples was validated using quantitative real-time PCR.
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Results of selected samples were validated using the Epitect Sequencing Service (Qiagen).
Results from clinical samples were validated using PCR.
The HBV integration sites identified in these samples were validated using PCR and Sanger resequencing [ 7].
CNVs detected in the UCL1 samples were validated using TaqMan® RNase P Copy Number Reference CNRR) Assay, Human (Applied Biosystems).
The samples were validated using data distribution and the sources of the variation parameters in the PGS).
After each step the samples were validated using an Agilent 2100 Bioanalyzer (Agilent Technologies), and the final concentration was measured using a Qubit 2.0 Fluorometer (Invitrogen).
The concentration of each sample was validated using the Nanodrop spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA).
The sampling method was validated using repeated rounds of sample collection to demonstrate that ≥ 95% of the organic and bioburden residuals were recovered in the first round of sample collection and that there was no added value in more extensive sampling.
The quantification of the phenolic compounds in virgin olive oils from different olive varieties was carried out by means of the standard addition method and, finally the procedure for the sample treatment was validated using the well established solid phase extraction procedure with Diol cartridges.
For the milk samples, the method was validated using cow milk as blank matrix.
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