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A cryo-cooled plasma cell for flat samples was used on the VG9000 instrument.
The same endogenous control (EC) sample consisting of a mix of 30 random mammary gland tumor samples was used on each blot.
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Blood samples are used on ELISA kit, to analyze CTX, TRACP 5b level.
Moreover, due to limited data samples are used on both sides, the estimation on two edges is very coarse.
After 5 fold dilution, the STA samples were used on a BioMark 96.96 Dynamic Array for Gene Expression, for determination of Ct (cycle threshold) values.
Triplicate samples of four leaf disks each were used on Day 0, and quintuplicate samples were used on Days 2 and 4. Error bars represent standard deviations of data means.
To assess statistical significance of classifier performance, a one-tailed t-test (significance level of alpha=0.05) for single sample was used on the performance level across subjects versus chance level (50%).
A total of 500 ng of RNA from each sample was used on an Illumina Sentrix Mouse Ref-8 Expression BeadChip (http://www.illumina.com/products/mouseref-8_v2_expression_beadchip_kit.ilmn) containing over 25,000 probes.
RDT testing from finger prick samples was used for on-site diagnosis and treatment of malaria.
One microliter of the digested samples was used to identify deglycosylated peptides on Bruker Anchorchip™ target.
Since a very small volume of sample is used on each grid, fibrils can be missed leading to false negative results.
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