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Residual DNA in the samples was removed using DNase I (Takara).
Subsequently, teeth and bones were irradiated with UV lamp for 15 min. Next, the external surface of the samples was removed using a sand-blaster to eliminate both soil and exogenous DNA contaminants.
Ribosome RNA of six RNA samples was removed using Ribo-Zero™ Magnetic Kit (Epicentre).
Genomic DNA contamination from the extracted RNA samples was removed using TURBO DNA-free kit (Ambion).
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The black materials on the surface of the samples were removed using a drill.
Shown are the running ground truth of the differential effect (black dotted line), the estimate when the designated number of samples is removed using the proposed pair-wise compatibility-based selection (blue line; see Section 2.2), and the estimate when the designated number of samples is removed using repeated random draws based sample selection (red line; see Section 2.4).
Color coordinates (L*a*b* values) useful for the color simulations were obtained using a fiber optic reflectance spectroradiometer model FS3 (ASD Inc., Boulder Co .. Selected samples were removed using a scalpel under high magnification.
Unincorporated dNTPs from the PCR samples were removed using a gel purification kit (Qiagen).
After incubation, the sperm in the dissociated testicular cell samples were removed using a modified two-gradient (35%70%%) Percoll method.
At regular intervals, slurry samples were removed, using an N2 degassed syringe, and analyzed for various geochemical parameters.
Three microliters of the sample was placed on the carbon-coated copper grid, making a thin film of sample on the grid, and an extra sample was removed using the cone of a blotting paper and kept in a grid box sequentially.
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