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Each tissue samples was pooled from multiple donors.
For cartilage, RNA from three independent cartilage samples was pooled and reverse transcribed.
To produce cDNA for standard curves, RNA from each of the control samples was pooled and serially diluted before cDNA synthesis.
An equal quantity of the cDNA from each of the different samples was pooled, ligated to EcoRI adaptors (5'-AATTCCGTTGCTGTCG-3', Promega #C1291) and digested with NotI.
RNA from replicate leaf samples was pooled prior to sequencing.
Five μg of RNA from each of the above five samples was pooled.
Similar(43)
The moose liver samples were pooled by the sampling area, sex and age.
Feces samples were pooled by cow.
Here, three treated samples were pooled and analyzed as one.
Milk samples were pooled by group and another milk sample was collected from the bulk tank.
Four samples were pooled and used for next generation sequencing using the 454-Roche protocol.
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