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Determination of p96 and p67 isoform expression levels in normal and breast tumour samples was performed utilising the Human Breast Cancer Rapid-Scan Panel (Origene) according to manufacturer's suggestions and performed in duplicate using the two individual plates provided.
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The samples were dewaxed and the DNA extraction was performed utilising a Stratagene Kit (Amsterdam, The Netherlands).
Statistical analysis was performed utilising GNU R [29].
A hybrid assembly [56] was performed utilising the Staden package [57], Phred [58], Phrap (http://www.phrap.org), Paracel (Paracel Inc).
Standardisation of DWI display was performed utilising b0 imaging.
Statistical analysis was performed utilising the online calculators of GraphPad Software.
Statistical analysis was performed utilising Student's t-test.
Subsequent analysis was performed utilising only the trough values.
qRT PCR was performed utilising the ABI prism 5700 sequence detection system (Applied Biosystems, Warrington, UK).
Monte Carlo simulations were performed utilising a Python script.
All analyses were performed utilising Statistical Package for Social Sciences 15.0.
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