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The protein content of the same samples was measured using Quick Start Bradford reagent (Bio-Rad).
The protein concentration of the samples was measured using the Bradford protein assay.
DNA concentration of the samples was measured using the Qubit® dsDNA BR Assay Kit (Thermo Fisher Scientific).
The concentration of the purified total RNA samples was measured using a Qubit RNA Assay Kit (Invitrogen) and read in a Qubit Fluorometer (Invitrogen).
The protein concentration of the samples was measured using the DC Protein Assay Kit (Bio-Rad, Hercules, CA, USA) with bovine serum albumin used as a standard.
The hydraulic conductivity of all samples was measured using ASTM method D4511-00.
Absorption of samples was measured using the surface thermal lensing (STL) technique.
The anode plasma electrolytic nitricarburising could decrease friction coefficient and increase wear resistance of thardnessarbof sthel.
Sound transmission loss (STL) of needled samples was measured using an impedance tube equipped with four microphones.
For normalization of ALP activity and calcium quantification data on Days 14 and 21, DNA content of samples was measured using the Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen) according to the manufacturer's instructions.
The absorbance of samples was measured using a UV-vis spectrophotometer.
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