Exact(10)
DNA extracted from the samples was initially amplified with universal 16S ribosomal DNA primers.
DNA extracted from the samples was initially amplified using universal 16S rDNA primers, followed by a second round of amplification using the first PCR products to detect a specific fragment of the 16S rDNA of each target Treponema species.
The effects of Li/Si molar ratio on the final products in phase composition and particle morphology were investigated, and the band gap energy of the as-prepared Li2SiO3 samples was initially estimated from UV visible spectra.
The effects of reaction time on the final products in phase composition and particle morphology were investigated, and the band gap energy of the as-prepared Li2SiO3 samples was initially estimated from UV Visible spectra.
Amount and the quality of the RNA samples was initially determined using NanoDrop™ 1000 spectrophotometer (NanoDrop Technologies, Inc., Wilmington, DE).
The molecular form of samples was initially determined using a combination of standard diagnostic assays (Favia et al. 2001; Fanello et al. 2003).
Similar(50)
Many S. chartarum samples were initially negative after PCR amplification.
Core samples were initially saturated with reservoir oil of 3.81 cp viscosity.
The samples were initially subjected to one cycle load up to 1% strain to determine the creep load.
Such samples were initially solidified at room temperature and then placed in an oven at 50 °C, 75 °C and 100 °C.
The fungal populations present in the samples were initially explored at the species level by mean of PCR-RFLP on the ITS1-5.8-ITS2 ITS1-5.8-ITS2 ITS1-5.8-ITS2
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