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Sub-analysis of studies comparing Xpert and culture on smear positive and negative samples was conducted.
Injection of 1 μL of samples was conducted in the splitless mode with a sampling time of 1.0 min.
The quality control of the mRNA samples was conducted using the Bioanalyzer 2100 (Agilent Technologies) and all samples had RIN values above 9.
Chemical analysis of these samples was conducted to determine the ion, metal, and organic and elemental carbon concentrations.
The oxidation resistance evaluation of the coated samples was conducted at 800 °C in air for 10 h.
Test for bacteria in the samples was conducted within 6 h after sampling.
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Radiocarbon dating (30 samples) was conducted by accelerator mass spectroscopy (AMS) at the Institute of Accelerator Analysis Ltd. and Geo Science Laboratory.
The isotopic analysis of greater covert samples was conducted at the University of California Davis Stable Isotope Facility (California, USA).
Statistical analysis to calculate the standard deviations for relative expression in different samples was conducted as described in the Applied Biosystems Taqman protocol handbook.
Duplicate genotyping of 5% of DNA samples was conducted with 100% success.
Culturing of blood samples was conducted at Chulalongkorn University in Bangkok, Thailand.
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