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Without large budgets, immigrant samples using random walk will result in poor sample quality.
cDNA was synthesized from 250 ng total RNA from the 8 cortex samples using random hexamers (Qiagen) and SuperScript™ II Reverse Transcriptase (Invitrogen).
cDNAs were synthesized from 0.4 μg of total RNA samples using random hexamer primers and Revert Aid H Minus cDNA synthesis kit (Thermo Scientific).
cDNA was synthesised from all 24 RNA samples using random hexamer primers and libraries were prepared by TruSeq RNA sample preparation kit (Illumina, San Diego, USA).
cDNAs were sythesized from 1 μg of total RNA samples using random primers and RevertAid H Minus cDNA synthesis kit (Fermentas).
For synthesis of cDNA 1ug of total epidermal RNA was used for epidermal samples using random hexamer primed M-MLV reverse transcriptase (Promega, Madison, WI).
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To facilitate abundance-based bacterial community comparison, we equilibrated sequence coverage by generating sequence subsets (n = 387 sequences per sample) using random sampling without replacement and performed taxonomic classification of sequence subsets using the Ribosomal Database Project (RDP) Naïve Bayesian Classifier [24].
Nor has there been a study of the SPADI in a population sample using random sampling.
Selection of controls employs a population sample using random digit dialing, and appropriately represents the entire study area.
First strand template cDNA was produced from 500 ng RNA for each sample using random primers (Invitrogen) and SuperScript III reverse transcriptase (Invitrogen) according to the manufacturer's protocol.
Cy3/Cy5-labelled cDNA was synthesized from 15 μg of the RNA sample using random hexamers and Superscript II reverse transcriptase (Invitrogen).
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