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This technique, which was readily designed for the clinical analysis of CD4+ lymphocytes in blood samples, uses a "volumetric cytometer" device.
Our method to quantify the amounts of proline (PRO) and HYP in different collagen samples uses a simple and standard reversed-phase liquid chromatograph coupled to a single analyzer time-of-flight MS (LC-MS) and requires no sample derivatization.
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DNA was isolated from blood samples using a salting-out method (after Lahiri and Schnabel, 1993).
Moreover, gene expression was profiled in the corresponding GPLs and EGC samples using a whole-genome microarray.
The landing on Phobos is planned for February 2013, followed by the collection of the soil samples using a claw on the end of a robotic arm.
They conducted a series of examinations using sophisticated microscopes and sliced samples using a focused-ion beam to check the internal structures.
Then, 100 ng of total RNA was used to quantify expression levels of miRNAs from animal blood samples using a probe-based miRNA expression assay (NanoString Technologies, USA).
DNA was extracted from daily samples using a DNeasy Plant Mini kits (Qiagen), with some modifications to the standard protocol that has been previously described34.
This analysis, in brief, was completed as previously described19 by debarcoding samples using a paired-end 2 × 8 bp barcode strategy.
Here, we demonstrate quaternary structure determination in biological samples using a combination of chemical cross-linking, high-resolution mass spectrometry and high-accuracy protein structure modeling.
The volume phase transition was observed as the change of the scattering intensities of the samples using a 532 nm probe laser.
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