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Comprehensive transcriptome studies of cancers often rely on corresponding normal tissue samples to serve as a transcriptional reference.
The earlier detection of a vast amount of microorganisms (from 105 to 108 cfu/g) within the raw vegetables led the possibility of those samples to serve as substrate to enhance the growth and replication of the spoilage microbes (Abadias et al. 2008; Cordano and Jacquet 2009; Rahman and Noor 2012).
Immunostaining for PTEN was first performed using normal brain and classic medulloblastoma tissue samples to serve as negative control and for optimization of the staining procedure, respectively, prior to using the TMA for IHC.
An additional GSEA was performed comparing the microarray data from the 24-hour control samples to that in the 72-hour control samples to serve as a negative control for changes in gene expression resulting from the culture conditions rather than exposure to forskolin.
Bovine serum albumin was added to samples to serve as an internal standard.
Transcripts of GAPDH and U6 were also detected from the same samples to serve as an internal control for normalization.
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The subjects in the control group were enrolled as a healthy convenience sample to serve as a comparison group for the measurement of mitochondrial respiration and ROS.
In general, non-parametric techniques select and copy regions from the input sample to serve as building blocks by pasting them together one at a time onto the outcome.
Meanwhile, GSO prepared a 15% master sample to serve as a future national survey-sampling frame.
A total of 60 designated (unauthorised) products were sampled to serve as controls.
There were 5582 (4.1%) people with medical service claims related to hypoglycaemia and 1 28 966 without, of whom 27 910 were randomly sampled to serve as controls in a 1 : 5 ratio.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com