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Preliminary reports revealed that a time of 10 min ensured sufficient relaxation to allow the samples to fully equilibrate [ 31].
Some methods involve in-silico analysis but some require additional samples from other affected family members or tumour samples to fully assess [ 12, 25, 27].
Future work will focus on the development of an internal amplification control for the assay and increasing the number of clinical samples to fully validate the RPA assay.
Future studies will need to focus on repeated testing of initial samples to fully establish the reliability and precision of these tests.
In view of the growing concern about occupancy of PCB-containing buildings, investigators should assess as full a profile of congeners as feasible in environmental and biological samples to fully characterize the nature and sources of PCB body burdens.
The cut-points determined in the training set need to be validated in an independent set of human patient samples to fully complete the establishment of this signature profile in OS.
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A 10-min time interval of the two consecutive indentations was set for the sample to fully recover prior to the next indentation.
Furthermore, large number of Pyrostria and bracteate species temporarily placed under Canthium s.l should be sampled to fully understand the evolutionary dispersal of the genus.
After measuring satisfaction over the course of 18 months, Byers concluded that sexual and relationship satisfaction do appear to change concurrently, but more complex models are necessary (e.g.: longer time period, more measurement points, larger sample, dyadic sample) to fully understand the connection.
We note, though, that it might take a very large sample to fully support this conclusion.
However, we suggest that elevation of independent lineages to specific status should only be done after an assessment of morphological variation, and detailed sampling to fully determine their distribution.
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