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This pilot study was completed by the 1000 Genomes Consortium to evaluate the use of LD information and sequence data from multiple samples to aid in the genotype calling from low coverage, whole genome sequencing (http://www.genome.gov/26524516).gov/26524516
Clinical laboratories have the primary (almost sole) responsibility of testing samples to aid clinical decision-making.
Fifty micrograms of Folsch-extracted cow brain lipids (Sigma) was added to all samples to aid recovery of phosphoinositides.
Infiltration times were increased to 48 h for Drosophila samples, to aid resin penetration through the abdominal cuticle.
Although PHLs also test samples to aid clinical decisions, functions like surveillance, strain identification, and tracking of drug resistance are arguably their main priorities.
Laboratories that are able to provide high-titer HEV samples to aid in development of the proposed panel are requested to contact the authors.
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Chlorophyll was removed from each sample to aid later imaging of GUS staining from the histochemical treatment.
Future work may also benefit some additional but targeted sampling to aid model calibrations.
Extracts were reduced by rotary evaporation, filtered, and composited into two equivalent POCIS samples, thereby increasing the amount of chemical present in each sample to aid in detection.
Anti-endotoxin antibody was added to the first sample to aid in the measurement of endotoxin, then the second sample is challenged with a maximum stimulatory dose of endotoxin (800pg/ml) to allow endotoxin quantitation and measure the state of neutrophil activation is assessed by the maximal chemiluminescent response.
A standard volume of 50 μl of CountBright beads (Invitrogen, Carlsbas, CA) was added to each pooled sample to aid in cell enumeration.
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