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Contrary to the fibre based samples, the well processed resin coated photographs aged at 50 °C and 90% RH darkened more than the insufficiently washed resin coated samples aged at both 70% RH and 90% RH.
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In all samples, the wells are separated from each other by wide GaAs barriers.
Although the genus Prevotella accounted for more than 25% of absolute abundance in all samples, the well-described P. ruminicola represented only about 1% of absolute abundance.
Based on the concentration of each metabolite in 600 μL (volume after dissolving of dried samples), the well-specific amount of each metabolite was calculated.
After removing the samples, the wells were washed with PBS-Tween (PBST) buffer for five times.
After incubation of the diluted samples, the wells were washed and subsequently incubated with 100 μL biotinylated antibody to CD18 (KIM127).
The exact weight/volume of the samples should be determined before placing the samples in the well counter.
Load the samples into the wells.
Depending on the volume of the sample well and the volume of the sample itself, the sample aliquot may not necessarily fill the entire well volume.
HH supervised the collection of the samples as well as the design of the study.
EMV supervised the collection of the samples as well as the design of the study.
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CEO of Professional Science Editing for Scientists @ prosciediting.com