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This design has an expected sample size of 6286%6% of the fixed sample value) and an expected study length of 3.1 years (77% of the fixed sample value).
The expected sample size under H0 is 53.2 (or 74% of the fixed sample value).
The fixed samples were concentrated to 20 30 ml.
The fixed samples were embedded in paraffin.
The fixed samples were decalcified using Cal-EX (Fisher Scientific), and embedded in paraffin for sectioning.
The fixed samples were washed in PBS containing 1% Triton X-100 (PBST).
The fixed samples were embedded in paraffin and serially sliced into 5-µm sections.
The fixed samples were homogenized using Precellys device (Bertin Technologies).
The samples were fixed on the stage of the goniometer.
The samples were fixed to the inside of the capsules using Omega CC high-temperature cement.
For that, the samples were fixed to the inner wall of a ball mill containing the zirconia balls.
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