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Many new analytical NMR applications have resulted from recent developments in the pharmaceutical industry that has changed the types of samples that require analysis.
As a result, this PCR provides robust detection of expanded alleles and resolves allele zygosity, thus minimizing the number of samples that require Southern blot analysis and producing more comprehensive FMR1 genotyping data than other methods.
This approach not only overcomes problems with incorrect results but also reduces the number of samples that require calculations.
For samples that require less than a total amount of 500 ml ethanol at concentrations lower than 80%, a cheaper transport service is available.
New tests based on PCR have been developed, being applied in samples that require less invasive procedures such as stool and NPA and showing to be useful especially in settings in whom GA are difficult to obtain.
Notably, and in contrast to ubiquitin, hydrazinolysis appeared to cease at approximately 40%% conversion after 48 hours, which is not an uncommon observation for samples that require the presence of guanidine⋅HCl (6 m) for sufficient solubility.
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Samples that required stabilization (the side wall core samples) prior to grinding, were stabilized.
Samples that required only one attempt at FNB had a sensitivity increase by >10% compared to samples with more than one attempt (z = 4.8, P < 0.001).
Samples that required chemotherapy to achieve return of hCG to baseline or total abdominal hysterectomy and bilateral salpingo-oophorectomy to treat locally invasive disease were classified as persistent.
In order to determine if pathogenic autoantibody responses are present in patients with adult onset multiple sclerosis, we developed a sensitive bioassay to identify demyelinating and/or axopathic autoantibodies in clinical samples that required no prior knowledge of their antigen-specificity.
For the samples that required saponification, the residue was dissolved in 2 ml of methyltert-butylether (MTBE), after which, 2 ml of a 15% (w/v) KOH/methanol solution was added for the saponification for 6 h in the dark under nitrogen [ 46].
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