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Finding samples that provide a good representation of sound events as defined in the taxonomy was more demanding.
It is important that biomarker samples are matched to samples that provide concurrent measurement of ALT and AST as well.
We have also collected whole blood samples that provide an unbiased representation of the transcriptomes from all blood cell types, including neutrophils.
We have made every effort to counterbalance this limitation by using a statistical analysis suitable for small sample sizes and by using novel and important samples that provide accurate in vivo data.
However, in vivo, most of these techniques are limited by the need to study whole kidneys for specific molecular events (as from tissue extracts), or to study events on histological samples that provide localization evidence, but more ambiguous molecular specificity.
Among gastrointestinal diseases with a possible microbial origin, NEC is somewhat unique as it is relatively common and because samples that provide information about gut consortia can be collected prior to the development of symptoms.
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We build an original industrial and technological database comprising various samples that provides quantitative information concerning KM and innovative practices.
The same approach has been applied to other ancient bones, including Neanderthal samples that provided around 1 million bp of its genome [9], as well as other Pleistocene mammals from Denisova cave in Siberia [10].
Interestingly, the eight ancient pig samples that provided PCR2 amplicons harboured Western haplotypes [see Additional file 3: Figure S2].
In most studies, samples that provided a specific diagnosis were considered TPs, given the high PPV ascribed to the technique [ 4, 5, 11, 17].
This is the proportion of the 500 samples that provided an estimate with a 95% C.I. containing the true population value [ 18, 22].
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