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The predominantly alkaline pH in reacted samples (Tab.
Tumour specific staining (Fig. 5) was observed in about 80% of the different tissue samples (Tab. 2), whereas an analysis of 272 non-tumour tissue sections revealed a very low reactivity with normal tissue (Tab. 3).
Association between age and expression of 4,502 age-associated genes (FDR ≤ 0.01) in 1,264 monocyte samples (Tab 1).
In DTT-treated samples, the amount of free F5 was 730 ± 140 % that of untreated samples (Tab. 2), demonstrating that both, H2O2 and DTT abolished DNA binding.
Ct-values (in case of detection) or 'N/A' (in case of non-detection), obtained with the iCycler iQ Real-Time PCR Detection System Software version 3.0a (Bio-Rad Laboratories, USA), have to be entered (copy-paste) in the 'Ct-values Samples' tab of the spreadsheet for all probes of all test samples, together with their 'Run ID' and 'Sample in', in order to generate the corresponding genotype.
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Because of the small size of the PS1 subunit, we chose to set the 'Perform image alignment' option on the Sampling tab to 'No'.
An 'Ace' file can be opened directly by ContigScape and the relationship of contigs can be saved as a CRS format (see sample, tabbed.txt, tabbedCov.txt, Additional file 1).
The program provides read counts for the different categories for all experimental and control samples as tab-delimited tables, along with the relevant p-values, indicating the statistical probability that the predicted mutation exhibits a normal expression pattern.
We further added more detailed information on the plant material used in each of the four affinity purification/kinase identification experiments that were conducted in the excel spreadsheet containing information on which kinase was identified in which sample (first tab of Figure 4 source data 1).
From the 'Results' tab, 'Sample ID' and genotype for every sample can be copy-pasted to other software programs, with a reminder if all control samples were genotyped correctly or not.
The genomic locations of the 137,899 unique exon junctions and the identities of the supporting reads from the A and B samples are provided in tab delimited format [see Additional file 3].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com