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None of these samples stimulated the production of SEAP from the 293XL-hTLR9 cellsuggestinging that the cfDNA from severe sepsis patients is host derived (data not shown).
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Samples stimulated under the same culture conditions in the absence of BFA showed undetectable cytokine levels.
Messenger RNA levels for genes of interest were measured by real-time PCR and samples stimulated in the absence or presence of CGS 21680 were compared in a time-matched manner.
For imaging of neurites in differentiating cultures, a rabbit polyclonal antitubulin IgG (Sigma, diluted 1 : 75 in 10% GS), or a rabbit monoclonal antineurofilament IgG (Millipore, diluted 1 : 200 in 10% GS) were used as primary antibodies after saturation on different sets of samples (stimulated with the same hypergravity conditions).
Saliva samples stimulated by mechanical stimulation produced significantly (p <0.05) higher salivary flow rate when compared with unstimulated resting drool.
Immunofluorescence mapping of white matter strips stimulated at 200 Hz demonstrated that βII spectrin diffused into nodal area (Fig. 6F) whereas in samples stimulated at 1 Hz the βII spectrin was restricted to paranodal and juxtaparanodal areas (Fig. 6G).
The principal component analysis (PCA; Figure 1) revealed that all samples stimulated by PMA-Ionomycin clustered according to the treatment and the time of stimulation (T4 or T24).
This suggests a similar potency of the two protocols to induce hormonal receptors and similar estrogenic capacity of the CC samples stimulated by HP-hMG and rFSH.
In total, 517 proteins were identified from at least two of the three independent samples stimulated with IL-1β.
The cutoff values for ELISpot and ICS were defined as the 99th percentile of 34 HIV-negative samples stimulated with 8 different peptide pools (272 different stimulations) [18].
The kinase activity in samples stimulated with GH (10 ng/ml) increased 5-fold compared to samples without addition of GH.
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