Figure 6 Effective spring constant as a function of sample stiffness.
Finally, these samples have strong adhesive properties which results in choosing cantilevers that possibly result in less contrast between the fibrils and the surface, because of the mismatch between cantilever and sample stiffness.
In addition, the boundary conditions that are given by Equations 9 and 10 correspond to the moment and the force balanced between the beam and a combination of the linear tip-sample stiffness at X = L, respectively.
Precise and accurate measurement of viscoelastic mechanical properties becomes increasingly challenging as sample stiffness decreases to elastic moduli <1 kPa, largely due to difficulties detecting initial contact with the compliant sample surface.
However, sample stiffness increased with further treatment cycles (P < 0.018, 4th cycle vs. native) (Fig. 5D).
We developed our own computational tools, written in Igor Pro (Wavemetrics, Portland, OR), to extract the sample stiffness from the raw force-volume files.
CPB caused two distinct changes in RBC elasticity; pre fix A indicates samples where stiffness increases or shows no change, B those where stiffness decreases.
After 4 months, they tested the samples for stiffness using a compression gauge.
For all samples storage stiffness increased with frequency.
