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Of five samples spot tested, samples identified to contain acrylic + CN tested positive for CN.
Two further sputum samples (spot and early morning sample) were collected for smear and culture and the TB suspect was transported to the tertiary referral hospital for chest x-ray examination.
Two or three sputum samples (spot or morning) were collected per patient in 50 ml sterile conical centrifuge tubes.
Variability in urine sample collection includes whether first morning void samples, spot samples, or total 24-hr voids are collected.
Baseline fasting blood samples, spot urine samples for assessment of the albumin-to-creatinine ratio, and 24-h urine collections were assayed using standard methods.
Three consecutive sputum samples (spot, early morning and spot) were taken from each patient according the Uganda National TB and Leprosy guidelines.
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Three consecutive sputum samples (spot-morning-spot) were collected and examined for the presence of Acid Fast Bacilli (AFB) using the standard Ziehl-Neelsen method[17].
Three sputum samples (spot-morning-spot) were collected from each participant.
Then, as previously described (16), Ziehl Neelsen staining was done on three sputum samples (spot-morning-spot) collected from each participant.
15 17 Three sputum samples (spot-morning-spot) were collected from 150 TB treatment naïve, consecutive patients with clinical diagnosis of pulmonary TB at the National TB and Leprosy Training Centre (NTBLTC), Zaria, Nigeria.
Three sputum samples (spot-morning-spot) were collected and smear microscopic examination for AFB was done at the respective health facilities according to the national TB and Leprosy control programme guideline [ 15].
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