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The distance of the light source from the upper level of the samples solution was 15 cm.
Where A (experiment) represents absorbance of wells with cells, CCK-8 solution and power samples solution; A (blank) represents absorbance of wells with medium and CCK-8 solution without cells and A (control) represents absorbance of wells with cells, CCK-8 solution without power samples solution.
The samples solution was placed in a cold water bath for 5 min, added with 100 μL of 1% (w/v) ascorbic acid solution and adjusted volume to 50 mL with methanol in a volumetric flask.
After removing old medium, the cells were treated with varying concentrations (0, 200 μg/mL) of test samples solution in MEM containing 1% FBS (MM) and incubated at 37°C for 3 days.
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R-Mandelic acid and l-alanine were obtained from Sigma Aldrich and were used as received; for other samples solution-phase optical rotation measurements or chiral separation established enantiomeric excesses of > 98%.
Photophysical performance comparison between solid state sample, solution sample and electrospinning fiber samples was performed.
A sample solution prepared by dialysis was used directly.
The sample solution was deposited dropwise onto a KBr disc.
20-μl sample solution is injected for the GPC analysis.
After about 5 h standing, the sample solution was completed.
The preconcentration procedure was applied to this sample solution.
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