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The data was collected under validated methodologies with quality control samples run with each batch.
For samples run with FID, compounds had been identified beforehand using GC-MS.
It should be noted that Figure 6 represents all patient samples taken through RT-PCR, including the 23 samples run with less than 1 ng/well input RNA.
Two Tecan Genesis liquid handling robots were used to assemble plates for 335 patient samples over a period of five weeks, for a total of 670 assay plates (this includes samples run with an RNA concentration of less than 1 ng/assay well).
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We performed 5,000 MCMC sampling runs with a leapfrog step size of 100.
Sample runs with spmoB were performed at protein concentrations of 0.3 30 μM.
We performed 5,000 MCMC sampling runs with a leapfrog step size of 100 to obtain the mean and 95%% confidence intervals for the threshold, slope, and lapse rate.
Within the amount of protein loaded on the gel, we detected only a single band in each sample running with the molecular mass corresponding to TBC1D4-short and TBC1D4-long.
Peptide conformations covering Z-components of end-to-end distances between 0.4 and 7.0 nm were chosen as starting structures for the umbrella sampling runs, with distance intervals of 0.4 nm.
Two independent experiments, with samples run in quadruplicate, were performed.
A total of fourteen batches of nineteen samples were run, with at least one sample run in triplicate per batch, and one sample repeated between batches.
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