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Samples run on CellSearch® were evaluated either at Genentech or at one of two reference laboratories as indicated in the figure legend.
For the 615 samples run on both SNP and CGH array platforms, we performed a sample-level 50% one-way overlap of stringent Agilent 1M CNVs with the stringent CNVs from the SNP array platforms.
The left and right panels display mass defect as a function of m/z for the same set of samples run on reverse phase or HILIC columns respectively.
Of the 1246 initial samples run on both panels, 87 (7.0%) specimens failed.
Samples run on SDS-PAGE were transferred to PVDF nylon membrane.
SDS loading buffer was added and samples run on a 6% Tris-glycine gel.
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The panels for MIC10 and QIL1 were the same samples ran on different gels.
Six samples were run on one array and the samples from the groups were randomly allocated.
Samples were run on one channel of the Illumina GAI.
Each sample was run on one eighth of a sequencing lane.
Concentrated supernatant protein samples were prepared as described above and samples were run on 10% gels.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com