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Two major demands limit its widespread use on biological samples: requirements for photoactivatable/photoconvertible fluorescent molecules, which are sometimes difficult to incorporate, and high background signals from autofluorescence or fluorophores in adjacent focal planes in three-dimensional imaging which reduces PALM resolution significantly.
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Sample volume: In comparison to MS, which generally requires only approximately 10 50 μL, H NMR sample requirements are much higher.
Fig. 9 Project requirement list Fig. 10 Sample requirements document.
This technique has been previously limited by large sample requirements.
Validation confirmed fulfilment of the equilibrium sampling requirements and high measurement precision.
Eligible participants were selected based on the inclusion criteria and sampling requirements.
The use of these technologies has the potential to reduce time and sample requirements.
The sensitivity and the small sample requirements make the assay suitable for high-throughput screening of biological samples.
Support vector regression raises much attention because of high generalization capability and lower training sample requirements (Widodo and Yang 2007).
Norway did not meet the sample requirements of TEDS-M, the response rate was less than 60%%.
In order to avoid azimuth image ambiguities, spatial sampling requirements have to be fulfilled along the aperture trajectory.
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