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Differentially abundant genes between both time points were selected for each diet using paired Wilcoxon test (P< 0·01), then clustered into metagenomic species (MGS), using the method based on binning co-abundant genes across all individuals samples reported previously.
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These animals form the bulk of the sample reported previously in studies of residual feed intake (RFI) [ 20, 23].
Analysis of both samples by Western blot indicated that the HSW contained more protein than that in the R samples as reported previously [ 19,)].
Total RNA was isolated from individual mucosal samples as reported previously [39] and suspended in a volume of 80 µl diethly pyrocarbonate-water.
More detailed information about the samples was reported previously [ 13].
Details of the extraction and cleanup of the air samples were reported previously by Dann (1998).
All analyzed tumor samples were reported previously in various studies [ 7, 22, 23, 31, 34, 36].
LRRK2 and DJ-1, but not α-synuclein, were detected in the urine exosome samples, as reported previously.
CNV regions (CNVRs) were determined by merging the overlapping CNVs identified in all samples, as reported previously [ 4].
For detection of dead cells, Evan's blue staining was directly performed on culture samples as reported previously [ 28].
To monitor the stepwise oxidation of the individual haems in PpcAM58 mutants, 2D-H-EXSY (exchange spectroscopy) spectra in the pH range 6 9 were acquired in partially oxidized samples, as reported previously for the wild-type protein [ 20].
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