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RNA-seq data of laser-capture microdissection (LCM) suprachiasmatic nucleus (SCN) samples recapitulate the cycling of clock gene expression and identify known and novel SCN enriched genes.
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We next performed a BRCA2 deficiency-targeted reanalysis of 150 metastatic PCa tumors, and each of the 18 BRCA2-mutated samples recapitulated the BRCA2 deficiency-associated mutation signature, underscoring the potent influence of these lesions on somatic mutagenesis and tumor evolution.
There is clear separation of SCC and AC in the cervix samples, recapitulating the qRT PCR data.
Hierarchical cluster analysis of all samples based on the probes with the highest variance across the 50 samples recapitulated the known hematopoietic differentiation pathway, with the exception that the NK-cell samples were more closely related to the Tc samples than Th.
First, mtDNA mutations that became recurrently fixed in several independent cancer samples recapitulated ancient mtDNA genetic backgrounds (haplogroups), thus suggesting commonalities in the selective constraints acting on the mitochondrial genome in cancer and during human evolution (Zhidkov et al. 2009).
Furthermore, neighbor-joining on the unique SOM-expression profiles constructed a tree of the samples that recapitulated the hematopoietic tree (Fig. 2d and Supplementary Fig. 2d).
We further showed that the averaged expression of the four selected genes evaluated by qRT-PCR using FFPE samples accurately recapitulates the prognostic performance of the GGI.
Moreover, Xn samples from NCAM+ cells can recapitulate the complex histology found in WT, including NCAM− descendent cells.
We have characterized a large number of different models and compared them to patient samples, but none of them fully recapitulate the complexity of the human condition.
Unsupervised hierarchical clustering revealed that the assay was able to accurately recapitulate the expected relationships among the different mixed samples (Fig. 3A).
In particular, disease genes are not an unbiased sample of the interactome, but are characterized by specific functional signatures that recapitulate the biology of the disorders.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com