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Further, TGA results reveal that, in SNPs incorporated samples, quantity of CH is higher (∼61% at 8 h) up to 10 h, and lower (∼32% at 24 h) from 15 to 24 h than the control, showing the nucleation and pozzolanic effect of SNPs respectively.
We checked RNA samples quantity and purity with a NanoDrop ND-1000 Spectrophotometer (NanoDrop, Wilmington, DE).
DNA samples quantity and quality were evaluated using a NanoDrop 1000 spectrophotometer (Thermo Fisher Scientific Inc., USA).
Samples quantity and integrity were verified by amplification of a 259-bp cyclophilin cDNA fragment using oligonucleotides CYC-F: 5'-TTTCACAGAAATTATTCCAGGGTTT-3' e CYC-R: 5'-CAATATTCATGCCTTCTTTCACTT-3'.
The initial concentration of Pdb was 1.2 mg/mL and NanA was 0.14 mg/mL; standard curves were constructed using band densitometry for each serial dilution and compared with band density in CSF samples (Quantity One software).
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To estimate this scaling factor, we compute the standard deviation of DNAm and mRNA Z-statistics over all genes (i.e. not just FEM genes) and samples, quantities we denote by σ Z (D) and σ Z (R).
When there is doubt, the law requires a "match flame test" of a sample quantity.
The difference in weight gives the sample quantity.
Finally, the influence of the extraction time and the sample quantity were also studied.
The calibration curves were defined for each compound in the range of sample quantity 0.02 0.5 μg.
Because of limitations in sample quantity, samples from each time point per treatment were pooled (n = 1 3 per treatment and time point) for analysis.
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