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For the remaining samples, purity and concentration were determined using spectrophotometry (Ultrospec 2000, Pharmacia Biotech, Bucks, UK).
The two species-specific rotifer samples (purity of >96%) differed significantly, in both their δC and δN values (Table S2; P < 0.05; t-test).
Absorbance was measured at 260 and 280 nm to give ratio of A260 A280 was 1.8 to 2.0 in range to determine the samples purity.
3. The two dominant rotifers, Brachionus plicatilis and Brachionus dimidiatus, were separated to single-species samples (purity >95%) and significantly differed in their isotopic values (4.1‰ in δC and 1.5‰ in δN).
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Purification strategy was designed, considering of the compound enrichment, sample purity and purification throughput.
Age calculations, including correction factors for fractionation and sample purity, are also discussed.
Further information of the sample purity and structure can be obtained from the Raman spectra.
Sample purity and CNT wall structure were determined by Raman spectroscopy, thermogravimetric analysis and magnetic measurements.
The dependence of perilymph sample purity on the location of sampling and on the volume withdrawn was quantified.
The sample purity was determined by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and Comassie blue staining.
Details of sample purity and composition, as provided by the supplier, include yield 99%; BaO/TiO2: 0.999–1.001; purity: 99.9%; APS: 100 nm (from scanning electron microscope); SSA: 10 11 m2/g; color: white; morphology: spherical and true density: 5.85 g/cm3.
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