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Plasma samples or cell supernatants (25 μl/well) were plated in triplicates.
The selection of genes included in the study was as follows: first, we selected genes previously described in ovarian carcinoma samples or cell lines with a role in the carcinogenesis process.
However, these studies were primarily designed to identify biallelic gene mutations by analyzing the residual non-deleted 5q31.2 allele using MDS samples, AML samples, or cell lines containing a cytogenetically visible 5q31.2 deletion, and they only examined 20 of the 28 5q31.2 genes [6], [7], [8], [9], [10].
Previous studies in MM evaluated survivin expression in tissue samples or cell lines [ 23– 28].
The Wilcoxon-matched paired test was used to assess differences in matched samples or cell populations.
Researchers typically prepare their own reference from pooled experimental samples or cell lines [ 7- 10].
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Spatially resolved transcriptomic methods determine both the sequence and the location of RNAs within tissue samples or cells.
These strategies are critically reviewed including current limitations as well as future perspectives for the design of efficient UCNPs especially for sensing application in biological samples or cells.
Whole exome sequencing (WES) or preferably whole genome sequencing (WGS) can be performed on DNA from blood samples or cells from other biological materials like mucosal cells from a buccal swab.
Total RNA was prepared from tissue samples or cells using an RNeasy Mini Kit (Qiagen, Inc, CA) following the manufacturer instructions.
Therefore, the database offers a Google-like web interface to search for cancer genome information on a single gene, sample or cell-line, and on multiple genes, samples or cell-lines.
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