Suggestions(2)
Exact(6)
MCMV DNA was detected in blood vessel samples of viral infected mice but not in the control mice by nested PCR assay.
A region approximately 1100 bases long was amplified from samples of viral DNA and fluorescently labeled with Cy5-modified dNTPs, and single-stranded DNA was prepared by strand separation.
In total, four samples of bacterial skin infections, two samples of viral skin infections, three samples of fungal skin infections and two samples of protozoic skin infections were investigated by HIF-1α-specific immunohistochemistry (Fig. 1).
In 1988, Shibata et al. [ 21] amplified 28 samples of viral isolates positive for CMV by PCR using IE and LA primers.
Samples of viral RNA were obtained based on the extraction of total RNA from the plasma using the Total RNA Purification Kit NORGENN, Biotek Corporation, Canada).
Since these two high-throughput sequencings were performed on samples of viral genomic DNA with different purity and concentration, the similarity of the two sequencing results therefore suggests that good results can be obtained even when the sample is contaminated with host DNA as long as there is sufficient coverage for accurate assembly.
Similar(54)
Sampling of viral RNA molecules from an extraction solution that follows Poisson distribution is subjected to the stochastic effects of sampling variation.
In addition, even better sampling of viral genomes may not allow explaining the presence of ORFans in some of the microbial genomes.
However, it is questionable whether a full sampling of viral genomes will provide homologs to 100% of the ORFans or only to a fraction of them.
As in the case of ORFans, this can be due to the extreme limited sampling of viral sequences (Daubin and Ochman 2004a, 2004b; Lerat et al. 2005).
We also used a Bayesian approach that identifies the proportion of the posterior sample of viral topologies that match the host phylogeny (e.g. [ 19]).
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Justyna Jupowicz-Kozak
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