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This approach was adapted for cDNA libraries [ 2] and, with the advent of capillary sequencing, became suitable for high-throughput sequencing of large samples of transcripts, termed Expressed Sequence Tags (ESTs).
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A national sample of transcripts found mathematics had twice as many F's and D's compared as other subjects.
The depressing conclusion of a faculty report: "failing math at all levels affects retention more than any other academic factor". A national sample of transcripts found mathematics had twice as many F's and D's compared as other subjects.
For example, we are developing real-time imaging and analytical methods to monitor progress of fire blight infection, with concurrent sampling of transcripts and the metabolome to identify specific spatio-temporal mechanisms at genetic, cellular, and molecular levels.
A sample of transcripts, and the putative proteins they encode, identified C43181_G1_I1 encoding a 293 nt long ORF with a predicted protein (molecular weight = 30 kDa) homologous to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Tables 2, 3a).
We think that, in the future, increased sampling of transcripts will lead to increased discovery of conservation of specific AS-NMD events between different organisms.
A random sample of transcripts was double-checked for accuracy.
This approach has a number of caveats, for example, it assumes unbiased sampling of transcripts.
Two researchers (GFB and KMT) independently read and coded a sample of transcripts.
Further, the sub-sample of transcripts mirrored the whole data set in this.
For reliability, another researcher (JB) read and coded a sample of transcripts.
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CEO of Professional Science Editing for Scientists @ prosciediting.com