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Samples of total RNA were extracted for miRNAs Affymetrix GeneChip miRNA 3.0 array analysis (Life Technologies).
Samples of total mixed ration and milk were collected on d 1, 2, and 10 to 14 of each period.
The samples of total lipid extract were evaporated to dryness in a round-bottom flask using a boiling water bath.
In the formula search, data from 50 randomly selected samples of total 80 samples were used for model training.
30 µl samples of total RNA were transcribed to cDNA with 600 U of reverse transcriptase.
Samples of total cell lysates were run on a reducing 7.5% SDS-PAGE and electroblotted onto nitrocellulose filter.
Using RNeasy Midi Kit (Qiagen, Valencia, Ca, USA), three or two independent samples of total RNA were isolated for each condition, respectively.
The accuracy of the microarray results were validated by analyzing 14 differentially expressed genes by qRT-PCR using the same samples of total RNA.
RNAi-mediated gene expression knockdown was determined in samples of total RNA isolated from MTs dissected three days after injection of AQP dsRNA.
We carried out RACE reactions on samples of total RNA collected from cells that had been infected with virulent and avirulent L. pneumophila.
Samples of total RNA (1.5 µg) were reverse transcripted with random hexamer primers using Multi-Scribe Reverse Transcriptase (TaqMan Reverse transcription reagents, Applied Biosystems, Foster City, CA).
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